What are the limitations of organoids as a model? Despite the promising features of organoids, their broad utility is tempered by a variety of limitations yet to be overcome, including lack of high-fidelity cell types, limited maturation, atypical physiology, and lack of arealization, features that may limit their reliability for certain applications.
What are the advantages of organoids? Organoids can be used for (1) basic research, including studies of human biology aiming to understand developmental processes, responses to external stimuli and stress signals, cell-to-cell interactions and mechanisms of stem cell homeostasis; (2) biobanking, whereby samples obtained from patients can be used to
What is the purpose of organoids? Organoids enable to study how cells interact together in an organ, their interaction with their environment, how diseases affect them and the effect of drugs. In vitro culture makes this system easy to manipulate and facilitates their monitoring.
What are the pros and cons of using organoids?
- Not an average culture.
- Organoid morphology.
- Purity and removing contaminants.
- Heterogeneity of organoids.
- Reproducibility and clonal drift.
- Evidence-based validation.